Manual of Medical Laboratory Techniques By S Ramakrishnan KN Sulochana

One can afford to lose wealth but not health which is a ‘must’ for happiness in life. No wonder ‘health for all’ is our goal . Strictly speaking, it should be the international goal too. For achieving this, diseases have to be erased from the Globe. It is impossible, as the cheapest commodity available at present appears to be only diseases! Hence, all attempts should be made to control and cure the diseases, to make the people happy—thanks to the medical and the technological professionals who strive and serve, day-in and day-out, devoting themselves to the care of the patients and alleviation of their sufferings. This book "Manual of Medical Laboratory Techniques By S Ramakrishnan KN Sulochana" will be the best quid for you to learn Laboratory Techniques easily.

Manual of Medical Laboratory Techniques By S Ramakrishnan KN Sulochana contents:

Part I: Organic

• Glucose, Glucose Tolerance Test  ,Urea  ,Creatinine ,Bilirubin , Cholesterol
• Low-density Lipoprotein Cholesterol (LDL)
• High-density Lipoprotein – Cholesterol (HDL)
• Triacylglycerols (Triglycerides)
• Total Proteins Albumin , Ceruloplasmin
• Cerebrospinal Fluid (CSF) Proteins
• Uric Acid ,Homocysteine ,Lactate ,Pyruvate
• Thiobarbituric Acid Reactive Substances (TBARS)
• Glutathione , Sodium, Potassium and Chloride
• Calcium , Phosphorus, Iron and Iron-binding Capacity(IBC)
• Vitamin A ,Vitamin E ,Vitamin C

Part II: Enzymes

• Aspartate Transaminase (AST) Serum Glutamic-Oxaloacetic Transaminase (SGOT)
• Alanine Aminotransferase (ALT)/Serum  Glutamic-Pyruvic Transaminase (SGPT)
• Alkaline Phosphatase (Bone Forming Enzyme)
• Acid Phosphatase , Acid Phosphatase (Tartrate-Labile) for Prostatic Cancer
• Angiotensin Converting Enzyme (ACE) ,Lactate Dehydrogenase (LDH)
• Glucose-6-Phosphate-Dehydrogenase
• Amylase ,Ornithine Aminotransferase (OAT) Manual of Medical Laboratory Techniques
• Glutathione Peroxidase (GPx) ,Superoxide Dismutase (SOD)

Part III: Electrophoresis

• Electrophoresis of Serum Proteins
• Electrophoresis of Serum Lipoproteins

Part IV: Chromatography

• Amino Acids ,2D Chromatography
• HPLC Analysis of Amino Acids

Part V: Inborn Errors of Metabolism

• Carbohydrates ,Glucose and Other Reducing Sugars
• Maple Syrup Urine Disease
• Cystine ,Tyrosine ,Homocystinuria ,Phenylketonuria

 Part VI: Collection of Test Sample

• Procedure for Blood Collection ,Collection of Urine Sample
• Collection of Stool Sample ,Fully Automated Chemistry Analyzers
• Disposal of Materials ,Quality Control

Unit 2: Genetics

• Introduction
• Cytogenetic Methods
• DNA Based Methods
• Quantification of Gene Expression
• Sterilization Procedures

Unit 3: Hematology and Clinical Pathology
Part I: Hematology

• Complete Blood Count ,Total Erythrocyte (RBC) Count
• Total WBC Count ,Differential Count Peripheral Smear Study
• Erythrocyte Sedimentation Rate ,Platelet Count Absolute Eosinophil Count
• Reticulocyte Count ,Red Blood Cell Indices ,Packed Cell Volume
• Hemoglobin Estimation (Cyanmethemoglobin Method)
• Hemoglobin Electrophoresis
• Blood Grouping and Rh Typing ,Direct and Indirect Coombs’ Test
• Activated Partial Thromboplastin Time Prothrombin Time
• Bleeding Time (Ivy Method) Clotting Time
•  Clot Retraction ,Fibrinogen Assay (Clot Weight Method)
• Euglobulin Lysis Time Test
• Factor XIII Assay  Examination of Malarial Parasite Examination of Microfilaria
• LE Cell Preparation ,Sickle Cell Preparation

Part II: Clinical Pathology

• Stool Examination Detection of Occult Blood in Stool
• Urine Examination—Complete Automated Method
• Qualitative Identification of Reducing Sugar in Urine by Benedict’s
• Test Qualitative Identification of Protein in Urine by Heat and Acetic Acid Method
• Qualitative Identification of Ketone Bodies in Urine
• Qualitative Detection of Bile Pigments by Fouchet’s Method
• Qualitative Detection of Bile Salts by Hay’s Test
• Qualitative Identification of Urobilinogen in Urine by Ehrlich AldehydeTest
• Qualitative Detection of Free Hemoglobin by Benzidine Test
• Detection of Bence Jones Protein (BJP)
• Analysis of CSF , Mantoux Test
• Microscopic Examination of Urine

Unit 4: Microbiology & Serology
Part I: Staining Techniques

• Gram Stain , KOH-Calcofluor White Stain , Ziehl-Neelsen Stain
• Concentration Method for Detection of Mycobacterium
• Modified Ziehl-Neelsen Stain  Manual of Medical Laboratory Techniques
• Giemsa Stain ,Nigrosin Stain ,Lactophenol Cotton Blue Stain
• Hanging Drop Method for Motility
• Immunofluorescence Staining—Chlamydia trachomatis
• Immunofluorescence Staining—For Viruses (HSV, CMV, VZV,Adenovirus)

Part II: Culture Methods

• Bacterial and Fungal Culture—Conjunctival Swab Semi-quantitative Method
• Bacterial and Fungal Culture—Conjunctival Scraping
• Bacterial and Fungal Culture—Lid Margin Swab Bacterial and Fungal Culture—Corneal 
• Scraping Bacterial and Fungal Culture—Intraocular Specimens
• Bacterial and Fungal Culture—Other Specimens
• Conventional Biochemical Tests for Identification of Bacterial Isolates 308
• Antibiotic Sensitivity , Acid Fast Bacilli (AFB) Culture
• Fungal Culture , Bacterial and Fungal Culture, Blood Culture and Sensitivity
• Processing of Cerebrospinal Fluid for Bacterial and Fungal Culture
• Bacterial Culture—Urine ,Bacterial and Fungal Culture—Throat Swab
• Maintenance of Cell Cultures ,Acanthamoeba Culture
• Chlamydia trachomatis Culture by Rapid Shell Vial Technique
• Virus Isolation by Conventional Tube Culture Method

Part III: Immunology 

•  Rheumatoid Arthritis Test  ,C-Reactive Protein
•  Serum Antistreptolysin ‘O’ (ASO) Titer • Serum Rapid Plasma Reagin (RPR) Test
•  Treponema Pallidum Hemagglutination (TPHA)
•  Brucella Agglutination Test  ,Qualitative Determination of HIV1/2 Antibody
• Rapid Detection of Hepatitis B Surface Antigen (HBs Ag)
• Elisa Test for the Detection of Antibodies to HIV-1 and HIV-2
• Western Blotting Technique—Confirmation Test for Detection of HIV–1/2 Antibodies
• Fluorescent Antinuclear Antibody Test (FANA)

Unit 5. Ophthalmic Histopathology

• Specimens Entry ,Fixation of Specimens
• Grossing Technique ,Decalcification
• Tissue Processing ,Embedding ,Paraffin Section Cutting